Surprise:Three different types of vitamin D can activate the vitamin D receptor – Oct 2013

Gene Expression Profiles in Human and Mouse Primary Cells Provide New Insights into the Differential Actions of Vitamin D3 Metabolites

PLoS ONE 8(10): e75338. doi:10.1371/journal.pone.0075338
Pentti Tuohimaa, Jing-Huan Wang, Sofia Khan, Marianne Kuuslahti, Kui Qian, Tommi Manninen, Petri Auvinen, Mauno Vihinen, Yan-Ru Lou mail

1α,25-Dihydroxyvitamin D3 (1α,25(OH)2D3) had earlier been regarded as the only active hormone.

The newly identified actions of 25-hydroxyvitamin D3 (25(OH)D3) and 24R,25-dihydroxyvitamin D3 (24R,25(OH)2D3) broadened the vitamin D3 endocrine system, however, the current data are fragmented and a systematic understanding is lacking. Here we performed the first systematic study of global gene expression to clarify their similarities and differences.

Three metabolites at physiologically comparable levels were utilized to treat human and mouse fibroblasts prior to DNA microarray analyses. Human primary prostate stromal P29SN cells (hP29SN), which convert 25(OH)D3 into 1α,25(OH)2D3 by 1α-hydroxylase (encoded by the gene CYP27B1), displayed regulation of 164, 171, and 175 genes by treatment with 1α,25(OH)2D3, 25(OH)D3, and 24R,25(OH)2D3, respectively. Mouse primary Cyp27b1 knockout fibroblasts (mCyp27b1−/−), which lack 1α-hydroxylation, displayed regulation of 619, 469, and 66 genes using the same respective treatments. The number of shared genes regulated by two metabolites is much lower in hP29SN than in mCyp27b1−/−. By using DAVID Functional Annotation Bioinformatics Microarray Analysis tools and Ingenuity Pathways Analysis, we identified the agonistic regulation of calcium homeostasis and bone remodeling between 1α,25(OH)2D3 and 25(OH)D3 and unique non-classical actions of each metabolite in physiological and pathological processes, including cell cycle, keratinocyte differentiation, amyotrophic lateral sclerosis signaling, gene transcription, immunomodulation, epigenetics, cell differentiation, and membrane protein expression.

In conclusion, there are three distinct vitamin D3 hormones with clearly different biological activities.
This study presents a new conceptual insight into the vitamin D3 endocrine system, which may guide the strategic use of vitamin D3 in disease prevention and treatment.

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441 genes regulated by each version of vitamin D, as well as their overlap on Venn diagram

Figure 2D – notice BRCA1

Genes regulated by 24R,25(OH)2D3in Cell Morphology, Cellular Function and Maintenance, DNA Replication, Recombination, and Repair.
Green indicates gene down-regulation and pink to red indicate gene up-regulation (the more intensive the color, the higher the expression level).

See great description of this study at Vitamin D Council

• Three versions of vitamin D can activate the vitamin D receptor in some cells Oct 2013

 Download the PDF from Vitamin D Life

See also Vitamin D Life

• Vitamin D Receptor Activator might help treat or prevent various diseases
• Search Vitamin D Life for 1α,25(OH)2D3 1170 hits as of April 2018
• Search Vitamin D Life for 24R,25(OH)2D3 1030 items as of April 2018
• Vitamin D and its active metabolites – DeLuca Jan 2014
• Vitamin D Receptor category has the following

384 studies in Vitamin D Receptor category

Vitamin D tests cannot detect Vitamin D Receptor (VDR) problems
A poor VDR restricts Vitamin D from getting in the cells
It appears that 30% of the population have a poor VDR (40% of the Obese )

A poor VDR increases the risk of 55 health problems  click here for details
The risk of 44 diseases at least double with poor Vitamin D Receptor as of Oct 2019

VDR at-home test $29 - results not easily understood in 2016
There are hints that you may have inherited a poor VDR

Compensate for poor VDR by increasing one or more:

Increasing	Increases
1) Vitamin D supplement   Sun, Ultraviolet -B	Vitamin D in the blood and thus in the cells
2) Magnesium	Vitamin D in the blood  AND in the cells
3) Omega-3	Vitamin D in the cells
4) Resveratrol	Vitamin D Receptor
5) Intense exercise	Vitamin D Receptor
6) Get prescription for VDR activator    paricalcitol, maxacalcitol?	Vitamin D Receptor
7) Quercetin (flavonoid)	Vitamin D Receptor
8) Zinc is in the VDR	Vitamin D Receptor
9) Boron	Vitamin D Receptor ?, etc
10) Essential oils e.g. ginger, curcumin	Vitamin D Receptor
11) Progesterone	Vitamin D Receptor
12) Infrequent high concentration Vitamin D Increases the concentration gradient	Vitamin D in the cells
13) Sulfroaphone and perhaps sulfur	Vitamin D Receptor

Note: If you are not feeling enough benefit from Vitamin D, you might try increasing VDR activation. You might feel the benefit within days of adding one or more of the above

Far healthier and stronger at age 72 due to supplements Includes 6 supplements which help the VDR

Comment by Vitamin D Life No, I do not understand this either