Enhancement in brain uptake of Vitamin D3 nanoemulsion for treatment of cerebral ischemia: Formulation, Gamma scintigraphy and efficacy study in transient middle cerebral artery occlusion rat models
Journal of Microencapsulation https://doi.org/10.1080/02652048.2020.1801870
Mukesh Kumar,Dhruv Kumar Nishad,Anoop kumar,Aseem Bhatnagar,Ritu Karwasra,Kushagra Khanna, show all
The study used radioactive Vitamin D
- Vitamin D nanoemulsion etc. for fortification, pills, injections, topical and cancer – July 2019
- Nanoemulsion vitamin D is again found to be the best liquid form (for rats in this case) – June 2019
- Inhaled nanoemulsion of Vitamin D killed lung bacteria – Sept 2017
- Vitamin D nanoemulsion, with comments on COVID-19 – June 2, 2020
- Bioavailability of nanoemulsion formulations of Vitamin D3 – Nov 2019
- Mucosal membranes (mouth, lungs, nose, intestines, etc) can activate Vitamin D – July 2020
Increased Vitamin D to the brain might help prevent/treat:
strokes, Alzheimer's, Parkinson's, Epilepsy, etc.
Normally the blood-brain barrier somewaht restricts Vitamin D from getting to the brain
The nose is directly connected to the brain – no blood-brain barrier
Founder of Vitamin D Life has been inhailing Vitamin D nanoemulsion through his nose and mouth since 2017
The lungs can activate vitamin D locally – a Vitamin D inhaler may help lungs – Aug 2016
Nanoemulsion Vitamin D may be a substantially better form
The nose, in addition to the lung, can activate Vitamin D locally.
A nose, surprisingly, has MORE square meters of surface area than a lung
Aim: For the treatment of cerebral ischemia, Vitamin-D3 loaded nanoemulsion were developed.
Method: Tween 20 and polyethylene glycol were choosen as surfactant/co-surfactant, while oleic acid as oil phase. The formulation was characterized for various in-vitro parameters. Targeting efficiency was investigated through radiometry, gamma scintigraphy and efficacy was studied in transient middle cerebral artery occlusion (MCAo) rat model.
Result: Vitamin D3-Nanoemulsion showed a mean size range of 49.29 ± 10.28 nm with polydispersity index 0.17 ± 0.04 and zeta potential 13.77mV. Formulation was found stable during thermodynamic stability study and permeated within 180 min through sheep nasal mucosa (permeation coefficient 7.873 ± 0.884 cm/h). Gamma scintigraphy and radiometry assay confirmed better percentage deposition (2.53 ± 0.17%) of 99mTc-Vitamin D3-Nanoemulsion through nasal route compared to IV administered 99mTc-Vitamin D3 solution (0.79 ± 0.03%). Magnetic Resonance Imaging (MRI) of the ischemic model confirmed better efficacy of Vitamin D3-Nanoemulsion.
Conclusion: This work demonstrated better permeation, deposition and efficacy of VitaminD3-Nanoemulsion through intranasal route.
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